1KX1
Rat mannose protein A complexed with Man6-GlcNAc2-Asn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-12-08 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.800, 255.560, 61.790 |
Unit cell angles | 90.00, 119.60, 90.00 |
Refinement procedure
Resolution | 37.040 - 2.800 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 37844 | |
Completeness [%] | 94.4 | 75.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 10% PEG 8000, 100mM Tris-Cl pH=8.0, 10mM NaCl, 20mM Cacl2, 2mM NaN3. Protein solution: 5mg/ml in 10mM Tris-Cl pH=8.0, 10 mM NaCl, 20mM CaCl2, 1.1mM Man6GlcNAc2Asn. VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | 2 (mM) | ||
2 | 1 | drop | Tris-Cl | 10 (mM) | pH8.0 |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 20 (mM) | ||
5 | 1 | drop | Man6GlcNAc2Asn | 1.1 (mM) | |
6 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
7 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.0 |
8 | 1 | reservoir | 10 (mM) | ||
9 | 1 | reservoir | 20 (mM) |