1KWK
Crystal structure of Thermus thermophilus A4 beta-galactosidase in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 97.592, 97.592, 128.898 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.620 - 2.200 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.20000 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.620 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | 0.325 |
Number of reflections | 36622 | |
<I/σ(I)> | 8.3 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, sodium acetate trihydrate, sodium cacodirate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |