1KWA

HUMAN CASK/LIN-2 PDZ DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	110
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1996-12
Detector	MARRESEARCH
Wavelength(s)	0.979, 1.033
Spacegroup name	C 2 2 21
Unit cell lengths	40.800, 97.000, 92.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.930
R-factor	0.251
Rwork	0.251
R-free	0.29800
Structure solution method	MAD, SIR
RMSD bond length	0.006
RMSD bond angle	23.800 *

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.032 *	0.293 *
Total number of observations	50697 *
Number of reflections	14216
<I/σ(I)>	21.6	3.1
Completeness [%]	88.4	78.1
Redundancy	3.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	21 *	PROTEIN WAS CRYSTALLIZED IN 1.8M AMMONIUM SULFATE AND 100MM MES PH 6.5 AT 21 DEGREES C., temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	hCASK	4 (mg/ml)
2	1	drop	ammonium sulfate	0.9 (M)
3	1	drop	MES	50 (mM)
4	1	reservoir	ammonium sulfate	1.8 (M)
5	1	reservoir	MES	100 (mM)