1KW2
CRYSTAL STRUCTURE OF UNCOMPLEXED VITAMIN D-BINDING PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 43 |
Unit cell lengths | 133.925, 133.925, 73.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 2.150 |
Rwork | 0.218 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | VITAMIN D-BINDING PROTEIN COMPLEXED WITH ACTIN PDB 1KXP |
RMSD bond length | 0.005 |
RMSD bond angle | 18.670 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.320 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.059 | 0.214 |
Total number of observations | 1041011 * | |
Number of reflections | 67175 * | |
<I/σ(I)> | 27.7 | 10.2 |
Completeness [%] | 94.9 | 81.2 |
Redundancy | 15.5 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 20 * | PEG 200, 28%, Sodium Acetate, 0.1M, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG200 | 28 (%) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |