1KVS

UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	87
Detector technology	AREA DETECTOR
Detector	SIEMENS HI-STAR
Spacegroup name	P 32 2 1
Unit cell lengths	83.500, 83.500, 108.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.188 *
Rwork	0.188
RMSD bond length	0.015
RMSD bond angle	16.200 *
Data scaling software	XSCALIBRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.150	2.150 *
Rmerge	0.064	0.245 *
Total number of observations	68358 *
Number of reflections	24177	3002 *
Completeness [%]	95.0	87 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9	4 *	pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	14-20 (%)
2	1	reservoir		500-750 (mM)
3	1	reservoir	CHES	50 (mM)