1KVB

E. COLI RIBONUCLEASE HI D134H MUTANT

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1993-05-13
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	41.790, 86.700, 37.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.197 *
Rwork	0.197
RMSD bond length	0.014
RMSD bond angle	0.029
Data reduction software	MADNES
Phasing software	X-PLOR (3.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.077
Total number of observations	28545 *
Number of reflections	9295
Completeness [%]	81.9	68 *
Redundancy	3.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *	macro-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	drop	Tris-HCl	0.2 (M)
3	1	reservoir	Tris-HCl	0.4 (M)