1KVA

E. COLI RIBONUCLEASE HI D134A MUTANT

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1993-05-29
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	43.950, 87.070, 35.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.197 *
Rwork	0.197
RMSD bond length	0.015
RMSD bond angle	0.031
Data reduction software	MADNES
Phasing software	X-PLOR (3.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800	1.800 *
Rmerge	0.074
Total number of observations	31418 *
Number of reflections	10270
Completeness [%]	77.1	59.4 *
Redundancy	3.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *	macro-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	drop	Tris-HCl	0.2 (M)
3	1	reservoir	Tris-HCl	0.4 (M)
4	1	reservoir	PEG6000	5 (%)