1KV7
Crystal Structure of CueO, a multi-copper oxidase from E. coli involved in copper homeostasis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000, 1.3772, 1.1922, 1.3804 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.820, 90.510, 53.090 |
Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.400 |
R-factor | 0.185 * |
Rwork | 0.185 |
R-free | 0.22100 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.026 |
Data scaling software | d*TREK |
Phasing software | MLPHARE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 1.490 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.064 * | 0.280 * |
Total number of observations | 733346 * | |
Number of reflections | 88826 * | |
<I/σ(I)> | 7 | 2.3 |
Completeness [%] | 98.5 * | 87.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 20% PEG, 200 mM ammonium acetate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 20 (%) | |
2 | 1 | reservoir | ammonium acetate | 200 (mM) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |