1KV7

Crystal Structure of CueO, a multi-copper oxidase from E. coli involved in copper homeostasis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000, 1.3772, 1.1922, 1.3804
Spacegroup name	P 1 21 1
Unit cell lengths	49.820, 90.510, 53.090
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	30.000 * - 1.400
R-factor	0.185 *
Rwork	0.185
R-free	0.22100
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.026
Data scaling software	d*TREK
Phasing software	MLPHARE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	1.490
High resolution limit [Å]	1.400	1.400
Rmerge	0.064 *	0.280 *
Total number of observations	733346 *
Number of reflections	88826 *
<I/σ(I)>	7	2.3
Completeness [%]	98.5 *	87.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	20% PEG, 200 mM ammonium acetate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	ammonium acetate	200 (mM)
3	1	reservoir	sodium acetate	100 (mM)	pH4.6