1KV2

Human p38 MAP Kinase in Complex with BIRB 796

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.000, 74.000, 76.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.228 *
Rwork	0.228
R-free	0.31800
Structure solution method	isomorphous to 1IAN
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.053 *
Total number of observations	22129 *
Number of reflections	8717
Completeness [%]	90.0	66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4	21-25 *	Pav, S., (1997) Protein Sci., 6, 242. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6.0 (mg/ml)
2	1	drop	inhibitor	1.4 (mM)
3	1	reservoir	PEG1500	28-32 (%(w/v))
4	1	reservoir	dithiothreitol	5 (mM)