1KV1

p38 MAP Kinase in Complex with Inhibitor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01-05
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	65.000, 74.400, 77.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.500
R-factor	0.221 *
Rwork	0.221
R-free	0.28400
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.046
Total number of observations	31423 *
Number of reflections	12621
Completeness [%]	94.0	60

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4	21-25 *	Pav, S., (1997) Protein Sci., 6, 242. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6.0 (mg/ml)
2	1	drop	inhibitor	1.4 (mM)
3	1	reservoir	PEG1500	28-32 (%(w/v))
4	1	reservoir	dithiothreitol	5 (mM)