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1KU7

Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9A
Synchrotron siteNSLS
BeamlineX9A
Temperature [K]93
Detector technologyCCD
Collection date2001-08-09
DetectorMARRESEARCH
Wavelength(s)0.979
Spacegroup nameP 21 21 21
Unit cell lengths36.180, 62.650, 94.810
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution25.000 - 2.400
R-factor0.255
Rwork0.255
R-free0.29300
Structure solution methodMAD
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMLPHARE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0002.490
High resolution limit [Å]2.4002.400
Rmerge0.052

*

0.197

*

Total number of observations29108

*

Number of reflections7801

*

<I/σ(I)>2.9
Completeness [%]97.797.7

*

Redundancy3.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

6

*

22.5

*

MES, PEG 4000, magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111MES
211PEG 4000
311magnesium acetate
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein6 (mg/ml)
21reservoirMES0.1 (M)pH6.0
31reservoirammonium sulfate2.4 (M)
41reservoir20 (mM)

217705

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