1KU1
Crystal Structure of the Sec7 Domain of Yeast GEA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.052, 75.881, 76.199 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.930 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 19.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.080 | 0.326 |
| Number of reflections | 52653 | |
| <I/σ(I)> | 14.6 | 2.7 |
| Completeness [%] | 97.0 | 94.4 |
| Redundancy | 4.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | used microseeding * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30-40 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1-4 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | or sodium citrate, pH5.0 |
