1KTZ

Crystal Structure of the Human TGF-beta Type II Receptor Extracellular Domain in Complex with TGF-beta3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	H 3 2
Unit cell lengths	114.410, 114.410, 209.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.000 * - 2.150
R-factor	0.201
Rwork	0.201
R-free	0.21500
Structure solution method	MIR
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.058	0.533
Total number of observations	154181 *
Number of reflections	29006
<I/σ(I)>	24.5	2.7
Completeness [%]	99.8	98.8
Redundancy	5.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *		HANGING DROP VAPOR DIFFUSION USING A WELL SOLUTION CONTAINING 20% (V/V) 2-METHYL-2,4-PENTANEDIOL AND 0.1 M CITRATE PH 4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	20 (%(v/v))
3	1	reservoir	citrate	100 (mM)	pH4.0