1KTV
Crystal Structure of Elongation Factor G Dimer Without Nucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.02916 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.987, 103.548, 176.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.730 - 3.800 |
Rwork | 0.287 |
R-free | 0.37400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1elo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.184 | 0.400 |
Number of reflections | 14546 | |
<I/σ(I)> | 3.5 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | PEG 8000, HEPES, Tris, Dithiothreitol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |