1KTP
Crystal structure of c-phycocyanin of synechococcus vulcanus at 1.6 angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-16 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 186.346, 186.346, 59.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.212 * |
Rwork | 0.216 |
R-free | 0.25400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7y |
RMSD bond length | 0.005 |
RMSD bond angle | 1.060 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.078 * | 0.378 * |
Total number of observations | 419135 * | |
Number of reflections | 51656 * | |
<I/σ(I)> | 10 | 3 |
Completeness [%] | 97.5 * | 99.3 * |
Redundancy | 5.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 22 * | Adir, N., (2001) J. Mol. Biol., 313, 71. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG4000 | 5 (%) | |
2 | 1 | drop | Tris | 50 (mM) | |
3 | 1 | drop | protein | 2.5-5 (mg/ml) |