1KTN
Structural Genomics, Protein EC1535
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-01 |
Detector | SBC-1 |
Wavelength(s) | 0.9795, 0.9798, 0.9650 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.566, 53.558, 81.365 |
Unit cell angles | 90.00, 109.97, 90.00 |
Refinement procedure
Resolution | 38.650 - 1.400 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.20500 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.078 | 0.309 |
Number of reflections | 98786 | |
<I/σ(I)> | 20 | 2.44 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 6 | 4.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 20% PEG4K, 0.2M MgCl2, 0.1M Tris_HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |