1KTI
BINDING OF 100 MM N-ACETYL-N'-BETA-D-GLUCOPYRANOSYL UREA TO GLYCOGEN PHOSPHORYLASE B: KINETIC AND CRYSTALLOGRAPHIC STUDIES
Replaces: 1K0QExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2001-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.380, 128.380, 116.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.710 - 1.970 |
Rwork | 0.191 |
R-free | 0.21900 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 2.000 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 66108 | |
<I/σ(I)> | 16.7 | 16.7 |
Completeness [%] | 95.6 | 95.6 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | BES, EDTA, 100 MM N-ACETYL-N'-BETA-D-GLUCOPYRANOSYL UREA, pH 6.7, SMALL TUBES, temperature 289K |