1KTI

BINDING OF 100 MM N-ACETYL-N'-BETA-D-GLUCOPYRANOSYL UREA TO GLYCOGEN PHOSPHORYLASE B: KINETIC AND CRYSTALLOGRAPHIC STUDIES

Replaces:  1K0Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-04-01
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 43 21 2
Unit cell lengths	128.380, 128.380, 116.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.710 - 1.970
Rwork	0.191
R-free	0.21900
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	2.000
High resolution limit [Å]	1.970	1.970
Number of reflections	66108
<I/σ(I)>	16.7	16.7
Completeness [%]	95.6	95.6
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	BES, EDTA, 100 MM N-ACETYL-N'-BETA-D-GLUCOPYRANOSYL UREA, pH 6.7, SMALL TUBES, temperature 289K