1KTE

CRYSTAL STRUCTURE OF THIOLTRANSFERASE AT 2.2 ANGSTROM RESOLUTION

Experimental procedure

Detector technology	AREA DETECTOR
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	23.440, 64.410, 29.500
Unit cell angles	90.00, 91.10, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.189
Rwork	0.189
R-free	0.26400
RMSD bond length	0.012
RMSD bond angle	2.700 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.200
Rmerge	0.056
Number of reflections	4467 *
Completeness [%]	99.0
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	reservoir	ammonium sulfate	60 (%sat)
3	1	reservoir	acetate	100 (mM)