1KTC
The Structure of alpha-N-Acetylgalactosaminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.054 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.930, 72.930, 176.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.400 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ktb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.379 |
| Total number of observations | 68982 * | |
| Number of reflections | 19336 | 1875 * |
| <I/σ(I)> | 15.6 | 4.1 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 293 | lithium sulfate, ammonium sulfate, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.6 (M) | |
| 2 | 1 | reservoir | lithium sulfate | 0.9 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.6 |
