1KT5
Crystal structure of bovine holo-RBP at pH 4.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.293, 48.769, 74.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.820 - 1.460 |
| R-factor | 0.1605 |
| Rwork | 0.149 |
| R-free | 0.22240 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.820 | 1.540 |
| High resolution limit [Å] | 1.450 * | 1.460 |
| Rmerge | 0.062 | 0.261 |
| Total number of observations | 136920 * | |
| Number of reflections | 28216 * | |
| <I/σ(I)> | 5.7 | 2.5 |
| Completeness [%] | 95.7 | 71.5 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 20 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 30 (mM) | pH7. |
| 2 | 1 | reservoir | sodium acetate | 20 (mM) | |
| 3 | 1 | reservoir | MPD | 8 (%(v/v)) | |
| 4 | 1 | reservoir | 0.5 (mM) |
