1KT2
CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO MOTH CYTOCHROME C PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | STORAGE PHOSPHORS |
| Collection date | 1996-01-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.790, 56.930, 115.990 |
| Unit cell angles | 90.00, 92.12, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.800 |
| R-factor | 0.221 * |
| Rwork | 0.220 |
| R-free | 0.29300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.397 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 * | 0.317 * |
| Number of reflections | 22827 | 3185 * |
| Completeness [%] | 95.2 | 93.4 |
| Redundancy | 2.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 298 | PEG4000, 2-propanol, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 12 (%) | |
| 3 | 1 | reservoir | 2-propanol | 12 (%) | |
| 4 | 1 | reservoir | sodium acetate | 60 (mM) | pH5.5 |
