1KSG

Complex of Arl2 and PDE delta, Crystal Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-21
Detector	MARRESEARCH
Wavelength(s)	0.782
Spacegroup name	P 1 21 1
Unit cell lengths	48.100, 45.700, 74.700
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	19.700 * - 2.300
R-factor	0.255 *
Rwork	0.255
R-free	0.30100
Structure solution method	combination of Molecular Replacement and SAD phasing
Starting model (for MR)	ARL3-GDP pdb entry 1fzq
RMSD bond length	0.010
RMSD bond angle	25.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.065 *	0.235 *
Number of reflections	14251
<I/σ(I)>	14.8	5.8
Completeness [%]	97.5	99.7
Redundancy	2.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	Renault, L., (2001) Acta Crystallogr., Sect.D, 57, 1167. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	9-12 (%)
2	1	reservoir	HEPES	0.1 (M)	pH7.0