1KSD

The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 6.5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-06-01
Detector	MAC Science DIP-2030K
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	89.251, 83.924, 60.878
Unit cell angles	90.00, 95.47, 90.00

Refinement procedure

Resolution	30.000 - 1.600
Rwork	0.170
R-free	0.20100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tf4
RMSD bond length	0.005
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.089	0.509
Total number of observations	654717 *
Number of reflections	57195
<I/σ(I)>	19.6	3.6
Completeness [%]	97.0	92.4
Redundancy	19.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5 *	291	Ammonium Sulfate, Sodium Citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	protein	0.1 (M)	pH4.5
3	1	drop	ammonium sulfate	0.8 (M)