1KSC

The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 5.6.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-06-01
Detector	MAC Science DIP-2030K
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	89.107, 83.943, 60.840
Unit cell angles	90.00, 95.60, 90.00

Refinement procedure

Resolution	30.000 - 1.550
Rwork	0.178
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tf4
RMSD bond length	0.005
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.500
High resolution limit [Å]	1.550 *	1.550 *
Rmerge	0.079	0.562 *
Total number of observations	1124141 *
Number of reflections	60004 *
<I/σ(I)>	19.6	3.6
Completeness [%]	93.3	83.8
Redundancy	18.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5 *	291	Ammonium Sulfate, Sodium Citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium citrate	0.1 (M)	pH4.5
3	1	drop	ammonium sulfate	0.8 (M)