1KRQ
CRYSTAL STRUCTURE ANALYSIS OF CAMPYLOBACTER JEJUNI FERRITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.966 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 152.050, 152.050, 152.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 8373 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 6.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | Ammonium sulfate, PEG400, Hepes, Sodium azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
