1KRN
STRUCTURE OF KRINGLE 4 AT 4C TEMPERATURE AND 1.67 ANGSTROMS RESOLUTION
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1992 |
| Detector | XUONG-HAMLIN MULTIWIRE MARK II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.440, 50.270, 32.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.680 * |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.23200 |
| RMSD bond length | 0.009 |
| Data reduction software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.680 * |
| Rmerge | 0.037 |
| Number of reflections | 7850 * |
| Completeness [%] | 81.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 * | CRYSTALLIZED FROM BASA (BENZYL AMIN SULFONIC ACID) BUT ONLY SULFATE VISIBLE IN THE STRUCTURE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | CAC | 50 (mM) | |
| 3 | 1 | drop | PEG4000 | 10 (%) | |
| 4 | 1 | reservoir | PEG | 22 (%) |
