1KRM
Crystal structure of bovine adenosine deaminase complexed with 6-hydroxyl-1,6-dihydropurine riboside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-12 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.54178 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.030, 80.030, 141.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.500 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24100 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.600 * |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.730 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.050 * | 0.187 * |
Number of reflections | 16108 * | |
Completeness [%] | 99.2 * | 100 * |
Redundancy | 8.9 * | 7.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 293 | Kinoshita, T., (1999) Acta Crystallogr.,Sect., D55, 2031. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.0-2.2 (M) | |
2 | 1 | reservoir | PEG400 | 2 (%(v/v)) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
4 | 1 | drop | protein | 20 (mg/ml) | |
5 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
6 | 1 | drop | purine riboside | 10 (mM) |