1KRB
CRYSTAL STRUCTURE OF KLEBSIELLA AEROGENES UREASE, ITS APOENZYME AND TWO ACTIVE SITE MUTANTS
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 170.800, 170.800, 170.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.900 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.095 |
| Number of reflections | 27404 |
| Completeness [%] | 98.0 |
| Redundancy | 3.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 25 * | Jabri, E., (1992) J.Mol.Biol., 227, 934. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | 1.6 (M) | ||
| 3 | 1 | drop | HEPES | 100 (mM) | |
| 4 | 1 | drop | 1.6 (M) | ||
| 5 | 1 | drop | urease | 10 (mg/ml) | |
| 6 | 1 | drop | Tris | 20 (mM) | |
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | drop | beta-mercaptoethanol | 1 (mM) |
