1KRA

CRYSTAL STRUCTURE OF KLEBSIELLA AEROGENES UREASE, ITS APOENZYME AND TWO ACTIVE SITE MUTANTS

Replaces:  3KAU

Experimental procedure

Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE MARK II
Wavelength(s)	1.5418
Spacegroup name	I 21 3
Unit cell lengths	170.800, 170.800, 170.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.19
Rwork	0.190
RMSD bond length	0.009
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.900
High resolution limit [Å]	2.300 *
Rmerge	0.155
Number of reflections	35214
Completeness [%]	98.0
Redundancy	7.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	25 *	Jabri, E., (1992) J.Mol.Biol., 227, 934. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir		1.6 (M)
3	1	drop	HEPES	100 (mM)
4	1	drop		1.6 (M)
5	1	drop	urease	10 (mg/ml)
6	1	drop	Tris	20 (mM)
7	1	drop	EDTA	1 (mM)
8	1	drop	beta-mercaptoethanol	1 (mM)