1KR4
Structure Genomics, Protein TM1056, cutA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793,0.9791,0.95200 |
| Spacegroup name | P 3 |
| Unit cell lengths | 52.237, 52.237, 33.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 1.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24080 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.056 | 0.416 |
| Number of reflections | 19451 | |
| <I/σ(I)> | 24.25 | 1.66 |
| Completeness [%] | 95.1 | 87.7 |
| Redundancy | 5.49 | 4.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | Zhang, R.G., (2001) Structure, 9, 1095. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.75 (M) |
