1KQX

Crystal structure of apo-CRBP from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	I 4
Unit cell lengths	88.759, 88.759, 38.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.060 - 1.700
R-factor	0.2032
Rwork	0.195
R-free	0.27600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	holo rat CRBPII
RMSD bond length	0.007
RMSD bond angle	1.900 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000 *	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.094	0.294
Total number of observations	143455 *
Number of reflections	16261 *
<I/σ(I)>	4.6	2.1
Completeness [%]	97.7	97.7 *
Redundancy	3.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	4 *	PEG 4000, Ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%(w/v))
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)	pH5.6
4	1	drop	protein	8.0 (mg/ml)