1KQW

Crystal structure of holo-CRBP from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	I 4
Unit cell lengths	89.018, 89.018, 38.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.989 - 1.380
R-factor	0.1654
Rwork	0.159
R-free	0.22700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1opb
RMSD bond length	0.009
RMSD bond angle	2.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.000 *	1.460
High resolution limit [Å]	1.380	1.380
Rmerge	0.046	0.234
Total number of observations	144066 *
Number of reflections	22248 *
<I/σ(I)>	8.1	2.9
Completeness [%]	82.4 *	72.3 *
Redundancy	3	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	4 *	PEG 4000, Ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%(w/v))
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)	pH5.6
4	1	drop	protein	8.0 (mg/ml)