1KQ7

E315Q Mutant Form of Fumarase C from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-08-10
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	104.020, 220.000, 86.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.179
Rwork	0.179
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fuo
RMSD bond length	0.007
RMSD bond angle	21.057 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	13.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.112
Number of reflections	24817
<I/σ(I)>	5.046
Completeness [%]	93.5 *
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.6 *	291	PEG 3350, citrate dithiothreitol, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	20 (mM)	pH7.5
2	1	1	dithiothreitol	5 (mM)
3	1	1	EDTA	5 (mM)
4	1	2	sodium citrate	150 (mM)	pH5.6
5	1	2	PEG3350	14 (%)