1KQ7
E315Q Mutant Form of Fumarase C from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-08-10 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.020, 220.000, 86.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fuo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.057 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 13.000 * |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.112 |
| Number of reflections | 24817 |
| <I/σ(I)> | 5.046 |
| Completeness [%] | 93.5 * |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5.6 * | 291 | PEG 3350, citrate dithiothreitol, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | 1 | dithiothreitol | 5 (mM) | |
| 3 | 1 | 1 | EDTA | 5 (mM) | |
| 4 | 1 | 2 | sodium citrate | 150 (mM) | pH5.6 |
| 5 | 1 | 2 | PEG3350 | 14 (%) |
