1KQ2
Crystal Structure of an Hfq-RNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.870, 115.600, 101.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.400 * - 2.710 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | one hexamer of 1KQ1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.380 | 2.890 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.064 * | 0.367 * |
| Total number of observations | 40717 * | |
| Number of reflections | 11950 | |
| Completeness [%] | 90.3 | 92.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | 298 | PEG 550, MgCl2, Hepes, KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 550 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | KCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | Hfq | 0.5-1 (mM) | ||
| 2 | 1 | RNA | 1 (mM) | ||
| 3 | 1 | 2 | PEG550 MME | 10 (%) | |
| 4 | 1 | 2 | 100 (mM) | ||
| 5 | 1 | 2 | 15 (mM) | ||
| 6 | 1 | 2 | Tris | 50 (mM) | pH7.5 |
