1KQ1

1.55 A Crystal structure of the pleiotropic translational regulator, Hfq

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-05-11
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	67.030, 89.950, 67.770
Unit cell angles	90.00, 97.95, 90.00

Refinement procedure

Resolution	22.400 * - 1.550
Rwork	0.237
R-free	0.25900
Structure solution method	SIR
RMSD bond length	0.013
RMSD bond angle	1.570 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.400 *	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.046 *	0.158 *
Total number of observations	460147 *
Number of reflections	111857 *
<I/σ(I)>	7.6
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	298	ammonium sulphate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		150 (mM)
2	1	1	Tris	25 (mM)	pH7.5
3	1	1	EDTA	0.5 (mM)
4	1	1	Hfq	20 (mg/ml)
5	1	2	ammonium sulfate	2.5 (M)
6	1	2	acetic acid	0.4 (M)	pH4.6