1KPT

STRUCTURE AND FUNCTION OF A VIRALLY ENCODED FUNGAL TOXIN FROM USTILAGO MAYDIS: A FUNGAL AND MAMMALIAN CALCIUM CHANNEL INHIBITOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-02-21
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	26.140, 63.760, 53.620
Unit cell angles	90.00, 92.20, 90.00

Refinement procedure

Resolution	6.000 - 1.900 *
R-factor	0.186 *
Rwork	0.174
R-free	0.23700 *
RMSD bond length	0.019 *
RMSD bond angle	1.950 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Rmerge	0.052
Number of reflections	15905
Completeness [%]	81.9 *
Redundancy	3.42 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.2 (M)
2	1	reservoir	isopropanol	20 (%)
3	1	reservoir	HEPES	0.1 (M)
4	1	drop	KP4 toxin	10 (mg/ml)
5	1	drop	sodium citrate	0.2 (M)
6	1	drop	isopropanol	20 (%)
7	1	drop	HEPES	0.1 (M)