1KOO
THE CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF A NOVEL RNA-BINDING DOMAIN FOUND IN THE HUMAN TAP NUCLEAR MRNA EXPORT FACTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2000-10-13 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 138.245, 138.245, 204.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.800 |
| R-factor | 0.317 * |
| Rwork | 0.317 |
| R-free | 0.39500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ft8 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.132 | 0.468 |
| Number of reflections | 32000 | |
| <I/σ(I)> | 9.8 | 2 |
| Completeness [%] | 95.5 * | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | sodium potassium tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | potassium/sodium tartrate | 1.1 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.05 (M) | pH5.6 |
