1KON
CRYSTAL STRUCTURE OF E.COLI YEBC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96404,0.97927,0.97941,0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.560, 73.480, 39.260 |
| Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.200 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.31700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 11468 | |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Tris-Cl, postassium chloride, dithiothreitol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
