1KOA

TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1995
Spacegroup name	I 21 3
Unit cell lengths	195.500, 195.500, 195.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.300
R-factor	0.251
Rwork	0.251
R-free	0.34250 *
RMSD bond length	0.015
RMSD bond angle	2.180
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	3.700 *
High resolution limit [Å]	3.300 *
Rmerge	0.157
Total number of observations	39131 *
Number of reflections	17405
Completeness [%]	83.4
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	Tris	20 (mM)
3	1	reservoir		200 (mM)
4	1	reservoir	dithiothreitol	2 (mM)