1KO8
Crystal structure of gluconate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | BRUKER SMART 1000 |
| Wavelength(s) | 1.093 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.790, 89.120, 51.560 |
| Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.254 * |
| Rwork | 0.254 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.940 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 * | 0.442 * |
| Total number of observations | 61464 * | |
| Number of reflections | 14896 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 17-20 (%(w/v)) | |
| 6 | 1 | reservoir | 1.4-1.7 (M) | ||
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
