1KO5
Crystal structure of gluconate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.845 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.274, 89.206, 51.479 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 * |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.27700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.049 * | 0.254 * |
| Total number of observations | 50986 * | |
| Number of reflections | 17024 | |
| <I/σ(I)> | 27.8 | 5.4 |
| Completeness [%] | 96.6 | 98.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 17-20 (%(w/v)) | |
| 6 | 1 | reservoir | 1.4-1.7 (M) | ||
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
