1KO3
VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-24 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.805, 78.040, 79.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.900 * |
Rwork | 0.209 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | VIM-2 oxidized PDB ID code 1KO2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.950 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.630 | 2.020 |
High resolution limit [Å] | 1.900 * | 1.910 |
Rmerge | 0.050 * | 0.140 * |
Number of reflections | 15030 | |
<I/σ(I)> | 12.6 | 5.4 |
Completeness [%] | 91.0 * | 50 |
Redundancy | 3.6 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | 30% PEG8K, 0.1M NaCac/Cac.acid, 0.2M Na acetate, 0.5mM TCEP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.5 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG8000 | 30 (%) | |
6 | 1 | reservoir | sodium acetate | 0.2 (M) | |
7 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
8 | 1 | reservoir | 10000 (nM) |