1KNZ
Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.275, 85.359, 96.051 |
| Unit cell angles | 89.99, 90.00, 90.05 |
Refinement procedure
| Resolution | 22.000 - 2.450 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 * | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.045 * | 0.149 * |
| Total number of observations | 1328873 * | |
| Number of reflections | 46429 | |
| <I/σ(I)> | 35 | 14 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 24 * | PEG 4000, ammonium sulfate, MES, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | MES/NaOH | 50 (mM) | pH5.4 |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | drop | protein | 5.5 (mg/ml) |
