1KNX
HPr kinase/phosphatase from Mycoplasma pneumoniae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.734, 127.845, 170.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.490* |
| R-factor | 0.224 |
| Rwork | 0.227 |
| R-free | 0.28000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.490 * | 2.490 |
| Rmerge | 0.068 | 0.418 * |
| Total number of observations | 347151 * | |
| Number of reflections | 91137 | |
| <I/σ(I)> | 22 | 3 |
| Completeness [%] | 97.1 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | Steinhauer, K., (2002) Acta Crystallogr., D58, 515. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 4 (%) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.6 |
| 4 | 1 | reservoir | 0.8 (M) | or NaCl | |
| 5 | 1 | drop | HPrK/P | 7 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 7 | 1 | drop | 0.6 (M) | ||
| 8 | 1 | drop | AMPPCP | 1 (mM) |
