1KNT
THE 1.6 ANGSTROMS STRUCTURE OF THE KUNITZ-TYPE DOMAIN FROM THE ALPHA3 CHAIN OF THE HUMAN TYPE VI COLLAGEN
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.700, 38.200, 28.800 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.600 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.450 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.500 * | 1.500 * |
| Rmerge | 0.073 * | |
| Total number of observations | 18393 * | |
| Number of reflections | 8388 | |
| Completeness [%] | 92.0 | 83 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 3 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | citric acid | 0.1 (M) | |
| 4 | 1 | reservoir | 0.074 (M) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.45 (M) |
