1KNM

Streptomyces lividans Xylan Binding Domain cbm13 in Complex with Lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.188, 46.203, 56.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.570 - 1.200
R-factor	0.126
Rwork	0.123
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.041 *	0.193 *
Number of reflections	30016
Completeness [%]	83.1	22.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	300	1.8 mM Ammonium Sulfate, 50 mM MES pH 6.5, 5% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	MES	50 (mM)	pH6.5
3	1	reservoir	dioxane	5 (%)
4	1	drop	protein	30 (mg/ml)