1KNL

Streptomyces lividans Xylan Binding Domain cbm13

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	55.953, 55.953, 97.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 1.200
R-factor	0.152
Rwork	0.139
R-free	0.16600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	1.230
High resolution limit [Å]	1.000 *	1.200
Rmerge	0.057 *	0.269 *
Total number of observations	573332 *
Number of reflections	65601 *
Completeness [%]	89.5	78.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	300	1.8 mM Ammonium Sulfate, 50 mM MES pH 6.5, 5% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	MES	50 (mM)	pH6.5
3	1	reservoir	dioxane	5 (%)
4	1	drop	protein	30 (mg/ml)