1KNI
Stabilizing Disulfide Bridge Mutant of T4 Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | rotating-anode X-ray tube |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 298 |
| Detector technology | photographic film |
| Collection date | 1991-07-01 |
| Detector | OSCILLATION CAMERA |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.300, 62.300, 95.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 1.700 |
| Structure solution method | Rigid body |
| Starting model (for MR) | WT* T4 lysozyme |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.900 |
| Data reduction software | ROTAVATA |
| Data scaling software | AGROVATA / ROTAVATA ((AGROVATA) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.071 |
| Number of reflections | 15778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 21.5 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 0.1 (M) | pH6.6 |
| 3 | 1 | drop | 0.55 (M) | ||
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | reservoir | Na/KPO4 | 1.9 (M) | pH7.1 |
| 6 | 1 | reservoir | 0.24 (M) | ||
| 7 | 1 | reservoir | beta-mercaptoethanol | 30-140 (mM) |
