1KNE
Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-30 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 33.745, 76.943, 75.947 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.620 - 2.400 |
R-factor | 0.246 * |
Rwork | 0.246 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 24.200 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.083 * | 0.297 * |
Number of reflections | 4086 * | |
<I/σ(I)> | 47.2 | 15.1 |
Completeness [%] | 99.5 * | 95 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.1 | 10 * | Ammonium Sulfate, MES, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | ||
2 | 1 | reservoir | MES | pH6.1 |