1KNA

Chromo domain of HP1 complexed with histone H3 tail containing dimethyllysine 9.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-10
Detector	BRUKER SMART 6000
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	33.792, 76.883, 75.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.100
R-factor	0.228 *
Rwork	0.228
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.079 *	0.279 *
Number of reflections	6032
<I/σ(I)>	61.6	10.5
Completeness [%]	99.8	99.3
Redundancy	20.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1 *	10 *	Ammonium Sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 283.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate
2	1	reservoir	MES		pH6.1